According to the minister, with this new case confirmed, the DRC is entering a new phase of the Ebola epidemic which now affects three health zones, including an urban health zone.
The country's Health Minister, Oly Ilunga Kalenga, confirmed a case in Mbandaka, a city of a million people about 130km (80 miles) from the area where the first cases were confirmed earlier this month.
The WHO is anxious about the disease reaching the city of Mbandaka with a population of about 1 million people, which would make the outbreak far harder to tackle.
Reports have further stated that over 40 people have been infected, while 23 have died so far.
"We will intensify the monitoring and tracing of the population to all air, sea and road access routes". "Since the announcement of the alert in Mbandaka, our epidemiologists are working in the field to identify people who have been in contact with suspected cases".More news: HTC is working on a next-gen 'blockchain phone'
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He said one sample proved positive for the deadly Ebola virus, bringing to three the number of confirmed Ebola cases in three health zones.
Health workers have recorded confirmed, probable and suspected cases of Ebola in three health zones of Congo's Equateur province, and have identified 432 people who may have had contact with the disease, the WHO said.
According to the country's Ministry of Health, the vaccine arrived on Wednesday, after it declared the outbreak in the northwest region near Congo-Brazzaville on May 8.
The WHO has also exported over 4000 doses of experimental vaccine to affected areas in Congo, with more aid expected soon, said the report.
More than 11,300 people were confirmed dead between March 2014 and January 2016 in Liberia, Guinea, Sierra Leone, Nigeria, the USA, and Mali.